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ent-Prostaglandin E2

Name: ent-Prostaglandin E2
Brand: TargetMol
SKU: T35991
Rating: 4.5 (1 reviews)

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Catalog No. T35991Cas No. 65085-69-0

Alias ent-Prostaglandin E2

Enzymatically-derived prostaglandin E2 (PGE2) is an optically pure compound whereas PGE2 derived from the free radical-catalyzed peroxidation of arachidonate is a racemic mixture. Ent-PGE2 is the opposite enantiomer of PGE2. Significant amounts of racemic PGE2 (rac-PGE2) are generated in vitro and in vivo in settings of oxidative stress via the isoprostane pathway. A proposed mechanism for the formation of rac-PGE2 involves the base catalyzed equilibration from 15-E2t-isoprostane (8-iso-PGE2), generated from the 15-H2t-isoprostane endoperoxide.

ent-Prostaglandin E2

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Catalog No. T35991Alias ent-Prostaglandin E2Cas No. 65085-69-0

Pack Size	Price	Availability	Quantity
100 μg	$110	35 days
500 μg	$195	35 days

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Product Introduction

Bioactivity

Description

Alias

ent-Prostaglandin E2

Chemical Properties

Molecular Weight	352.471
Formula	C₂₀H₃₂O₅
Cas No.	65085-69-0
Relative Density.	no data available

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

PBS (pH 7.2): 2 mg/mL (5.67 mM), Sonication is recommended.

DMF: 50 mg/mL (141.86 mM), Sonication is recommended.

Ethanol: 50 mg/mL (141.86 mM), Sonication is recommended.

DMSO: 50 mg/mL (141.86 mM), Sonication is recommended.

Solution Preparation Table

PBS (pH 7.2)/DMF/Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8371 mL	14.1856 mL	28.3711 mL	141.8556 mL
5 mM	0.5674 mL	2.8371 mL	5.6742 mL	28.3711 mL

DMF/Ethanol/DMSO

	1mg	5mg	10mg	50mg
10 mM	0.2837 mL	1.4186 mL	2.8371 mL	14.1856 mL
20 mM	0.1419 mL	0.7093 mL	1.4186 mL	7.0928 mL
50 mM	0.0567 mL	0.2837 mL	0.5674 mL	2.8371 mL
100 mM	0.0284 mL	0.1419 mL	0.2837 mL	1.4186 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

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