Gly-Pro-AMC hydrobromide (Gly-Pro-AMC HCl) is a specific fluorescent dye used in cytofluorescence assays to detect Dipeptidyl peptidase IV (DPP-IV) activity.

Gly-Pro-AMC hydrobromide, at a concentration of 50 µM and under incubation conditions of 20 minutes at 37 °C, serves as a specific fluorescent substrate for the detection of DPP-IV activity[2].The fluorescence properties of Gly-Pro-AMC hydrobromide can be detected under the following conditions: excitation wavelength at 350 nm and fluorescence emission spectrum detection at 450 nm[2].