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HBV-IN-4

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HBV-IN-4

Catalog No. T36747Cas No. 2305897-84-9

HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].

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Pack Size	Price	Availability
5 mg	$297	6-8 weeks
25 mg	$916	6-8 weeks
50 mg	$1,190	6-8 weeks
100 mg	$1,860	6-8 weeks

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Product Introduction

Bioactivity

Description	HBV-IN-4, a derivative of phthalazinone, functions as a potent, orally active inhibitor of HBV DNA replication, exhibiting an IC50 value of 14 nM. It effectively induces the formation of genome-free capsids, demonstrating significant anti-HBV potency[1].
Targets&IC50	HBV DNA replication:14 nM (IC50)
In vitro	HBV-IN-4 (compound 19f; 0-1 μM; 8 days) effectively inhibits relaxed circular [rc] and single-stranded [ss] HBV DNA in a dose-dependent manner in HepG2.2.15 cells. It also decreases capsid-associated DNAs and promotes genome-free capsids, particularly those with accelerated migration phenotype[1].
In vivo	HBV-IN-4 (Compound 19f; 50-150 mg/kg; oral administration; twice a day; for 4 weeks; Balb/c male mice) achieves a 2.67 log viral load reduction in the AAV-HBV/mouse model[1]. It exhibits favorable drug characteristics, including low plasma clearance (CL=4.1 mL/min/kg), excellent drug exposure (AUC0-t=49,744 h·ng/L), T1/2 (2.15 hours), and oral bioavailability (F=60.4%) with 20 mg/kg oral administration in mice. HBV-IN-4 also shows good liver exposure distribution[1].

Chemical Properties

Molecular Weight	479.89
Formula	C24H19ClFN5O3
Cas No.	2305897-84-9

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 100 mg/mL (208.38 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0838 mL	10.4191 mL	20.8381 mL	104.1905 mL
5 mM	0.4168 mL	2.0838 mL	4.1676 mL	20.8381 mL
10 mM	0.2084 mL	1.0419 mL	2.0838 mL	10.4191 mL
20 mM	0.1042 mL	0.5210 mL	1.0419 mL	5.2095 mL
50 mM	0.0417 mL	0.2084 mL	0.4168 mL	2.0838 mL
100 mM	0.0208 mL	0.1042 mL	0.2084 mL	1.0419 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

HBV-IN-4HBV IN 4HBVIN4