Hoechst 33342 analog trihydrochloride, an analog of Hoechst 33342, serves as a fluorochrome by binding to the minor groove of DNA, facilitating the visualization of cellular DNA.

Hoechst 33342 selectively interacts with adenine-thymine-rich areas within the DNA's minor groove and serves as a marker for the nuclear DNA of cultured cells. It is detectable through imaging techniques, utilizing an excitation wavelength (λ ex) of approximately 353 nm and an emission wavelength (λ em) of about 483 nm for Hoechst 33342[1].