QHow to store the inhibitor solutions?
AAfter the stock solution is prepared, it is typically stored at -80°C and can be kept for over 1 year. It is recommended to aliquot to avoid repeated freeze-thaw cycles. For frequent use, it can be stored at 4°C for over a week.
QWhat should I do if the IC50 obtained for cell killing are significantly different from that presented on the your website?
AThe IC50 you obtain refers to the cell proliferation inhibition assay (half maximal inhibitory concentration), which is different in meaning from the IC50 on our website. The IC50 often refers to the inhibition rate of the target without cell experiments, which is generally determined through kinase or protein purification experiments, and is commonly reported in nanomolar concentrations. However, the IC50 obtained from cell proliferation inhibition assays refers to the half-maximal lethality of the cells, which involves cellular metabolism and penetration, so the concentration is usually higher.
Furthermore, the effect of the same compound on different cell models varies. It is recommended to try increasing the incubation volume and extending the incubation time.
QWhat should I do if I find that the ice pack has melted upon receiving the product?
AGenerally, powders are not sensitive to the temperature and the ice pack is used to prevent extreme temperatures. The melt ice pack will not affect the product quality.
QWhat are the common methods for dissolving compounds in animal experiments?
AFirst, you need to confirm the dosage and administration route. For specific products, it is recommended to refer to literatures that cite our product. If there are no relevant references and the compound dissolves well in DMSO, we recommend a general formula: 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline/PBS/ddH2O. The solvents should be added sequentially, and each solvent should be added after ensuring as much dissolution as possible. For normal mice, keep the DMSO concentration below 10%, and for nude mice and mice with weak tolerance, the DMSO concentration should be kept below 2%. The ratio of PEG300 and Tween-80 can be adjusted accordingly based on whether the solution is clear or not. Other co-solvents can also be used.
The above formula is for reference only. Please adjust according to different conditions. It is recommended to first test a small amount of compound with the formula before preparing a large quantity. Additionally, sonication or heating can be used to accelerate dissolution.
For intraperitoneal injection, it is preferable to use salt forms of compounds as they have higher solubility. If the dosage is large, suspension may be used for intraperitoneal administration.
For oral gavage, especially with larger doses, it is recommended to prepare a homogeneous suspension using 0.5% CMC-Na.
QHow to deal with the insoluble impurities in the product?
AInsoluble impurities, which do not affect the product activity, are recommended to filter out or remove. We will investigate to confirm whether the impurity was introduced during packaging or if it is inherent to the product itself.