QHow to set the concentration of stock solution?
AThe concentration of stock solution should be lower than the solubility provided on the official website. Within this range, the concentration of stock solution should be set based on that of working solution. For cell experiments, it is recommended to set the stock solution concentration at least 1000 times higher than that of working solution.
QAnimals cannot tolerate DMSO well. How should the dosage of DMSO be controlled during administration?
AFor normal mice, the concentration of DMSO should be kept below 10%. For nude mice, transgenic mice, or mice with weak tolerance, the concentration of DMSO should be kept below 2%. For inhibitors being used for the first time, it is recommended to perform a solvent-negative control experiment to confirm that the solvent has no nonspecific effects on the animals.
QHow to store inhibitor products?
ASolid-form products can be stored at -20°C for more than 3 years.
Stock solutions are typically stored at -80°C and can be stored for more than 1 year. It is recommended to aliquot the product to avoid repeated freeze-thaw cycles.
For short-term storage, samples can be kept at 4°C for more than one week.
QCan the inhibitor DMSO stock solution be directly diluted with buffer to create a gradient?
AIn most cases, it can dissolve. However, sometimes organic reagents may precipitate when directly added to an aqueous medium. It is recommended to first dilute the inhibitor with DMSO to form a gradient, and then add the diluted inhibitor to the buffer or cell culture medium. Some inhibitors may only dissolve in the aqueous phase at their working concentrations.
For example, if a final concentration of 1 μM is desired in cell experiments, the 10 mM DMSO stock solution can be diluted to 1 mM with DMSO, and then 2 μL can be drawn and added to 2 mL of saline/PBS/cell culture medium, resulting in a final concentration of 1 μM.
To avoid precipitation of the drug, it is recommended to preheat the mother liquor and culture medium to 37°C before dilution to avoid serious precipitation caused by low temperature. If precipitation occurs during the dilution process, it is suggested to use ultrasonic heating to redissolve the compound.
QWhat should I do if I find that the ice pack has melted upon receiving the product?
AGenerally, powders are not sensitive to the temperature and the ice pack is used to prevent extreme temperatures. The melt ice pack will not affect the product quality.