L-ascorbic acid 2-phosphate can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-ascorbic acid 2-phosphate increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation. L-ascorbic acid 2-phosphate is a long-acting vitamin C derivative that can stimulate collagen formation and expression.

A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect .?L-Ascorbic acid 2-phosphate (50 μM-250 μM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity.?The highest proliferation, ALP activity and runx2 expression is achieved with 150 μM AsA2-P and 10 nM dexamethasone (Dex), and 250 μM AsA2-P and 5 nM Dex.L-Ascorbic acid 2-phosphate ?significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) ?achieves only weak growth stimulation.