L-Methionine γ-lyase (Methionine lyase), a multifaceted enzyme in the γ-family of pyridoxal-5’-phosphate (PLP)-dependent enzymes, catalyzes the α, γ-elimination of L-methionine and homocysteine. This enzyme exhibits anticancer and anti-infectious properties and is utilized in homocysteinemia research [1][2][3].

L-Methionine γ-lyase (Methionine lyase) demonstrates anticancer activity against liver cancer cell lines (Hep-G2 and HCT-116) at concentrations up to 1000 μg/mL over 24 hours [1]. Furthermore, it exhibits cytotoxicity toward colorectal, hepatocellular, ovarian adenocarcinoma, and lung adenocarcinoma cancer cells, with IC50 values ranging from 0.05 to 2.14 Unit/mL [2].

L-Methionine γ-lyase (Methionine lyase), administered subcutaneously at a dose of 3 × 10^10 CFU over four days, attenuated P. gingivalis-induced periodontitis in BALB/c mice [3].