Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) is a minor component of the central nervous system myelin that induces potent T-cell and B-cell responses, is highly brain-derived and immunogenic, and causes a relapsing-remitting neurological disease with widespread plaque-like demyelination. [1]

METHODS: Dendritic cells (DCs) were loaded with MOG35-55 peptide at a concentration of 10 μg/mL. Two hours later, lipopolysaccharide (LPS, Sigma-Aldrich) was added to the cultures at a concentration of 0.25 μg/mL. Two days later (day 8 of culture), DCs were phenotyped using flow cytometry.
RESULTS In cultures of DCs loaded with Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)), the cytokines secreted were IL-6, IL-10, IFN-γ, TNF-α, and TGF-β, with TGF-β secreted at the highest concentration; IL-4 and IL-17 were not detected in any of the cultures. [2]

METHODS: Female C57BL/6 mice (n = 20) were subcutaneously injected with 200 μL of 200 μg Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)). 14 days after immunization, T cells were isolated from the spleen and purified into CD4 and CD8 T cells using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis; these cells were stimulated with Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) and used in proliferation assays. Enzyme-linked immunosorbent assay (ELISA) was used to detect interferon-γ (IFN-γ) and interleukin (IL)-4 secretion from the supernatants of cultured CD4 and CD8 T cells.
RESULTS After CD8/CD4 enrichment, CD8CD3 and CD4CD3 cells accounted for 86% and 94% of the total CD3 cells, respectively. CD8 T cells secreted less IFN-γ and IL-4 than CD4 T cells. [1]

Female C57BL/6 mice (n?=?20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide.?At 14 days after immunization, T cells were isolated from the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis.?These cells were stimulated by MOG35-55 peptide and applied to proliferation assays.?The interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA).?For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells.?EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining[1].