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N-Aminofluorescein

🥰Excellent
Catalog No. T86956Cas No. 98907-26-7

N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).

N-Aminofluorescein

N-Aminofluorescein

🥰Excellent
Catalog No. T86956Cas No. 98907-26-7
N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).
Pack SizePriceAvailabilityQuantity
2 mg28 €In Stock
5 mg39 €In Stock
10 mg55 €In Stock
25 mg102 €In Stock
50 mg169 €In Stock
100 mg248 €In Stock
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Product Introduction

Bioactivity
Description
N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).
In vitro
N-Aminofluorescein consists of a fluorescein molecule and a hydrazide group that recognizes and binds to Cu2+, thereby promoting hydrolysis of the amide. [1]
N-Aminofluorescein (abbreviated as FG) is specific for Cu2+, and an absorption peak at 632 nm is observed in 70% HEPES buffer (pH 7.4) containing Cu2+. absorption peak and emission peak was observed at 515 nm. [2]
Chemical Properties
Molecular Weight346.34
FormulaC20H14N2O4
Cas No.98907-26-7
SmilesO=C1C=2C=CC=CC2C3(C4=CC=C(O)C=C4OC5=CC(O)=CC=C53)N1N
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO: 100 mg/mL (288.73 mM), Sonication is recommended.
Solution Preparation Table
DMSO
1mg5mg10mg50mg
1 mM2.8873 mL14.4367 mL28.8734 mL144.3668 mL
5 mM0.5775 mL2.8873 mL5.7747 mL28.8734 mL
10 mM0.2887 mL1.4437 mL2.8873 mL14.4367 mL
20 mM0.1444 mL0.7218 mL1.4437 mL7.2183 mL
50 mM0.0577 mL0.2887 mL0.5775 mL2.8873 mL
100 mM0.0289 mL0.1444 mL0.2887 mL1.4437 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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