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N-Aminofluorescein

Name: N-Aminofluorescein
Brand: TargetMol
SKU: T86956
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).

Catalog No. T86956Cas No. 98907-26-7

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N-Aminofluorescein

Catalog No. T86956Cas No. 98907-26-7

N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Pack Size	Price	Availability
2 mg	$30	In Stock
5 mg	$42	In Stock
10 mg	$58	In Stock
25 mg	$108	In Stock
50 mg	$178	In Stock
100 mg	$262	In Stock

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Product Introduction

Bioactivity

Description	N-Aminofluorescein is a highly selective and sensitive fluorescent probe for Cu2+ and can be used to detect Cu2+ in biological systems (λex/em=495/516 nm).
In vitro	N-Aminofluorescein consists of a fluorescein molecule and a hydrazide group that recognizes and binds to Cu2+, thereby promoting hydrolysis of the amide. [1] N-Aminofluorescein (abbreviated as FG) is specific for Cu2+, and an absorption peak at 632 nm is observed in 70% HEPES buffer (pH 7.4) containing Cu2+. absorption peak and emission peak was observed at 515 nm. [2]

Chemical Properties

Molecular Weight	346.34
Formula	C₂₀H₁₄N₂O₄
Cas No.	98907-26-7

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 100 mg/mL (288.73 mM), Sonication is recommended.

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.8873 mL	14.4367 mL	28.8734 mL	144.3668 mL
5 mM	0.5775 mL	2.8873 mL	5.7747 mL	28.8734 mL
10 mM	0.2887 mL	1.4437 mL	2.8873 mL	14.4367 mL
20 mM	0.1444 mL	0.7218 mL	1.4437 mL	7.2183 mL
50 mM	0.0577 mL	0.2887 mL	0.5775 mL	2.8873 mL
100 mM	0.0289 mL	0.1444 mL	0.2887 mL	1.4437 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More Dose Conversion

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc