Nitro Blue Diformazan serves as a multifunctional dye, invaluable in biological experiments for observing cell structures, tracking biomolecules, evaluating cell functions, distinguishing cell types, detecting biomolecules, studying tissue pathology, and monitoring microorganisms. Its applications span from fundamental scientific research to clinical diagnostics. Additionally, dyes play a crucial role in traditional areas like textile dyeing and are increasingly used in emerging fields such as functional textile processing, food pigments, and dye-sensitized solar cells.