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p-Nitrophenylphosphorylcholine

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Catalog No. T37434Cas No. 21064-69-7

p-Nitrophenylphosphorylcholine is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.

p-Nitrophenylphosphorylcholine

p-Nitrophenylphosphorylcholine

😃Good
Catalog No. T37434Cas No. 21064-69-7
p-Nitrophenylphosphorylcholine is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.
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Product Introduction

Bioactivity
Description
p-Nitrophenylphosphorylcholine is a chromogenic substrate that is used to measure phospholipase C (PLC) activity. Hydrolysis of p-nitrophenylphosphorylcholine by PLC results in the liberation of p-nitrophenol, which can be measured at 405 nm at pH 7.2-7.5.
Chemical Properties
Molecular Weight304.24
FormulaC11H17N2O6P
Cas No.21064-69-7
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
PBS (pH 7.2): 10 mg/mL (32.87 mM), Sonication is recommended.
DMF: 1 mg/mL (3.29 mM), Sonication is recommended.
DMSO: 10 mg/mL (32.87 mM), Sonication is recommended.
Ethanol: 10 mg/mL (32.87 mM), Sonication is recommended.
Solution Preparation Table
DMF/PBS (pH 7.2)/DMSO/Ethanol
1mg5mg10mg50mg
1 mM3.2869 mL16.4344 mL32.8688 mL164.3439 mL
PBS (pH 7.2)/DMSO/Ethanol
1mg5mg10mg50mg
5 mM0.6574 mL3.2869 mL6.5738 mL32.8688 mL
10 mM0.3287 mL1.6434 mL3.2869 mL16.4344 mL
20 mM0.1643 mL0.8217 mL1.6434 mL8.2172 mL

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
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Keywords

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