Name: R110
Brand: TargetMol
SKU: T60744
Rating: 4.5 (1 reviews)

Pack Size

Price

Availability

Quantity

25 mg

$1,170

6-8 weeks

50 mg

$1,530

6-8 weeks

100 mg

$2,500

6-8 weeks

Qwhat frequency should sonication be used?

AIt is recommended to use the conventional ultrasound cleaning frequency

QCan PEG400 be used instead of PEG300?

APEG300 has moderate viscosity, which facilitates the dissolution and administration of drugs, while also providing good tolerability without adverse effects on animals. PEG400 can replace PEG300. PEG600 is not very recommended because its melting point is close to room temperature.

QCan Tween-80 be used instead of Tween-20?

ATween-20 is not recommended. Tween-80 has better tolerance and has been used as a tool to assess the behavioral effects of experimental drugs and toxins without significant side effects. Reference: Castro CA, Hogan JB, Benson KA, Shehata CW, Landauer MR. Behavioral effects of vehicles: DMSO, ethanol, Tween-20, Tween-80, and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.

QAnimals cannot tolerate DMSO well. How should the dosage of DMSO be controlled during administration?

AFor normal mice, the concentration of DMSO should be kept below 10%. For nude mice, transgenic mice, or mice with weak tolerance, the concentration of DMSO should be kept below 2%. For inhibitors being used for the first time, it is recommended to perform a solvent-negative control experiment to confirm that the solvent has no nonspecific effects on the animals.

QHow much inhibitor should be added in cell experiments?

AIt is recommended to refer to literatures of experiments conducted using the same model. In addition, it is also necessary to determine the optimal working concentration (concentration gradient) through pre-experiments by constructing a dose-response curve. Similarly, the optimal incubation time (time gradient) should be determined through a time-course experiment. Moreover, the amount of inhibitor used is influenced by various factors, including the accessibility of the target, cell permeability, incubation time, cell type, and others. We recommend determining the initial concentration of the inhibitor by referring to the literatures. If the Ki or IC50 are reported, it is recommended to start with 5-10 times their reported values to achieve the optimal inhibition of enzyme activity. If the Ki or IC50 are unknown, the inhibitor concentrations should be tested, and the Ki value can be calculated using Michaelis-Menten kinetics. Typically, the solvent used for dissolving the inhibitor is used as a control to eliminate the non-specific effects of the solvent.

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.