RhlR antagonist 1

RhlR antagonist 1 is a highly effective antagonist of the RhlR protein, showing an IC50 value of 26 μM. It exhibits selective antagonistic activity against RhlR, with minimal effect on LasR and PqsR. RhlR antagonist 1 strongly inhibits biofilm formation in both static and dynamic environments and reduces the production of virulence factors, such as rhamnolipid and pyocyanin, in P. aeruginosa. These characteristics make RhlR antagonist 1 a valuable tool for the development of molecules that modulate quorum sensing and control P. aeruginosa infections [1].

Catalog No. T60249

Resource Download

RhlR antagonist 1

Catalog No. T60249

All TargetMol products are for research purposes only and cannot be used for human consumption. We do not provide products or services to individuals. Please comply with the intended use and do not use TargetMol products for any other purpose.

Pack Size	Price	Availability
25 mg	$1,520	10-14 weeks
50 mg	$1,980	10-14 weeks
100 mg	$2,500	10-14 weeks

Bulk & Custom

Add to Cart

Questions

Product Introduction

Bioactivity

Description	RhlR antagonist 1 is a highly effective antagonist of the RhlR protein, showing an IC50 value of 26 μM. It exhibits selective antagonistic activity against RhlR, with minimal effect on LasR and PqsR. RhlR antagonist 1 strongly inhibits biofilm formation in both static and dynamic environments and reduces the production of virulence factors, such as rhamnolipid and pyocyanin, in P. aeruginosa. These characteristics make RhlR antagonist 1 a valuable tool for the development of molecules that modulate quorum sensing and control P. aeruginosa infections [1].
In vitro	RhlR antagonist 1 (compound 30) at 10 μM significantly impedes P. aeruginosa biofilm formation by 74%, concurrently reducing carbohydrate and protein content by 39% and 72%, respectively, and markedly curtails rhamnolipid synthesis at both 10 and 100 μM. A 24-hour exposure to 0-10 μM levels notably suppresses rhlA gene expression in biofilm cells. Thus, this compound effectively obstructs P. aeruginosa biofilms and virulence factor production by down-regulating rhlA expression in P. aeruginosa.
In vivo	Larvae administered with P. aeruginosa began experiencing mortality within the initial incubation phase, resulting in a 70% mortality rate by the conclusion of the 20-day period. Treatment with RhlR antagonist 1 (0-10 μM) significantly enhanced survival, with about 80% of larvae surviving by the period's end. Moreover, larvae inoculated with rhlR mutants of P. aeruginosa exhibited a 90% survival rate after 20 days [1].

Chemical Properties

Molecular Weight	208.2
Formula	C₁₂H₁₀F₂O
Cas No.

Storage & Solubility Information

Storage

Shipping with blue ice.

Calculator

Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Molecular Weight Calculator

In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

You can also refer to dose conversion for different animals. More

Tech Support

Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc