SHP2-IN-8 is a reversible, noncompetitive, selective and efficient allosteric SHP2 inhibitor (IC50=23 nM, Ki=22 nM), which also has effects in cells. SHP2-IN-8 can cause significant thermal displacement（ Δ Tm = 7.01 ℃）。 SHP2-IN-8 can inhibit the phosphorylation of AKT and induce apoptosis of Hela cells.

SHP2-IN-8 (compound TK-453), at concentrations from 0 to 100 μM over 48 hours, shows moderate inhibitory effects on KYSE-70, Hela, and THP-1 cell proliferation. At 0 to 30 μM for 24 hours, it induces dose-dependent apoptosis in Hela cells. Within 0 to 2 hours at 0 to 30 μM, SHP2-IN-8 inhibits AKT phosphorylation in Hela and KYSE-70 cells in a concentration- and time-dependent manner. These effects were confirmed using Cell Proliferation Assay, Apoptosis Analysis, and Western Blot Analysis, highlighting the compound's impact on cell growth, apoptosis, and AKT pathway inhibition in the tested cell lines [1].