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Streptavidin is a protein derived from the bacterium Streptomyces avidinii, with a molecular weight of approximately 60 kDa as a homologous tetramer. Streptavidin is known for its strong affinity to bind with biotin (Vitamin H or B7). The interaction between biotin and streptavidin is one of the strongest non-covalent interactions known in nature, with a dissociation constant (KD) of approximately 0.01 picomolar. Streptavidin also has immunosuppressive properties.
Description | Streptavidin is a protein derived from the bacterium Streptomyces avidinii, with a molecular weight of approximately 60 kDa as a homologous tetramer. Streptavidin is known for its strong affinity to bind with biotin (Vitamin H or B7). The interaction between biotin and streptavidin is one of the strongest non-covalent interactions known in nature, with a dissociation constant (KD) of approximately 0.01 picomolar. Streptavidin also has immunosuppressive properties. |
In vitro | Compared to the control group, streptavidin (2-200 μg/mL) significantly inhibits the proliferation of Lewis T cells towards WF, with an inhibition rate of 76%-83% [1].
In Western blot or ELISA, a concentration of 0.1-1 µg/mL is sufficient. For immunoprecipitation, a concentration of 1-10 µg/mL can be used. |
In vivo | Compared to the control group, streptavidin (2-200 μg/mL) significantly inhibits the proliferation of Lewis T cells towards WF, with an inhibition rate of 76%-83% [1].
In Western blot or ELISA, a concentration of 0.1-1 µg/mL is sufficient. For immunoprecipitation, a concentration of 1-10 µg/mL can be used. |
Cas No. | 9013-20-1 |
Relative Density. | 1.31g/cm3 |
Storage | keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
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