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TCEP-biotin
Catalog No. T36392Cas No. 2227020-26-8
TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation. Covalent addition of the TCEP moiety to crotonylated lysine residues results in adduct formation that allows for detection or immobilization of crotonylated proteins through interaction with the biotin ligand. It has been used in the detection and isolation of crotonylated histones in HEK293 cells.
TCEP-biotin
TCEP-biotin
Catalog No. T36392Cas No. 2227020-26-8
TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation. Covalent addition of the TCEP moiety to crotonylated lysine residues results in adduct formation that allows for detection or immobilization of crotonylated proteins through interaction with the biotin ligand. It has been used in the detection and isolation of crotonylated histones in HEK293 cells.
| Pack Size | Price | Availability | Quantity |
|---|---|---|---|
| 1 mg | $601 | Backorder |
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Product Introduction
Bioactivity
Chemical Properties
| Description | TCEP-biotin is biotinylated form of the reducing agent TCEP and an affinity probe for protein lysine crotonylation. Covalent addition of the TCEP moiety to crotonylated lysine residues results in adduct formation that allows for detection or immobilization of crotonylated proteins through interaction with the biotin ligand. It has been used in the detection and isolation of crotonylated histones in HEK293 cells. |
| Cas No. | 2227020-26-8 |
Storage & Solubility Information
| Storage | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. |
| Solubility Information | DMSO: 25 mg/mL Ethanol: 25 mg/mL DMF: 25 mg/mL PBS (pH 7.2): 0.15 mg/mL |
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In Vivo Formulation Calculator (Clear solution)
Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . A total of 10 animals were administered, and the formula you used is 5% 
DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.Preparation method for in vivo formula: Take 50 μL DMSO main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarify
main solution, add 300 μLPEG300 mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O mix well and clarifyFor Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
Dose Conversion
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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc
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