TMX-4100 is a potent degrader of phosphodiesterase 6D (PDE6D), exhibiting high degradation selectivity towards PDE6D with DC50 values below 200 nM in MOLT4, Jurkat, and MM.1S cells. This compound holds significant potential for research pertaining to multiple myeloma.

TMX-4100 (compound 3; 1 μM; 4 h) demonstrates a significant preference for degrading PDE6D in MOLT4 cells[1], exhibiting superior proteome-wide degradation selectivity compared to the PDE6D degrader FPFT-2216[1]. Importantly, TMX-4100 does not inhibit the growth of KRAS-dependent cell lines (MIA PaCa-2, NCI-H358, AGS, PA-TU-8988T)[1]. Western Blot analysis conducted on MOLT4 cells at a concentration of 1 μM for 4 hours confirmed TMX-4100's high PDE6D degradation preference[1]. Further analysis across MOLT4, Jurkat, and MM.1S cell lines, with concentrations ranging from 0 nM to 1 μM and a 4-hour incubation, revealed a consistent preference for PDE6D degradation, achieving a DC 50 value of less than 200 nM[1].