TMX-4116, a casein kinase 1α (CK1α) degrader, preferentially degrades CK1α in MOLT4, Jurkat, and MM.1S cells, exhibiting degradation concentration 50s (DC50s) below 200 nM. TMX-4116 is utilized in the research of multiple myeloma [1].

TMX-4116, at a concentration of 1 μM for 4 hours, demonstrates a significant preference for degrading CK1α in MOLT4 cells, as confirmed by Western Blot Analysis. At a lower concentration of 250 nM, TMX-4116 specifically induces the degradation of CK1α without affecting the levels of PDE6D, IKZF1, and IKZF3 in the same cell line. Further analysis revealed that TMX-4116 exhibits a strong selective degradation of CK1α across different cell lines, including MOLT4, Jurkat, and MM.1S, with a DC50 value of less than 200 nM, highlighting its efficacy over a range of concentrations.