Tubulin polymerization-IN-24 (compound HMBA) is a potent inhibitor of tubulin polymerization, increasing GTP hydrolysis rate and inhibiting microtubule assembly. It induces apoptosis and cell cycle arrest in the G2/M phase, and inhibits the proliferation of MCF-7 cells [1].

Tubulin polymerization-IN-24 (compound HMBA) effectively impedes the assembly of purified, microtubule-associated proteins-free tubulin in a concentration-dependent manner, evidenced by the inhibition of MCF-7 cell proliferation with an IC50 of 47 nM and the competitive obstruction of colchicine binding to tubulin, with a K i of 3.6 μM. Additionally, it disrupts mitotic progression in MCF-7 cells, leading to p53 pathway-mediated apoptotic cell death [1].