Tubulin polymerization-IN-26 (compound 12h) is a chemical compound that inhibits the polymerization of microtubulin. It achieves this by binding to the colchicine binding site of microtubulin, with an IC50 value of 4.64 μM. Additionally, Tubulin polymerization-IN-26 has the ability to induce apoptosis and inhibit cell metastasis or migration. Due to these properties, it holds potential as a valuable compound for researching lung cancer [1].

Tubulin polymerization-IN-26 (compound 12h), with concentrations ranging from 0.27-30 μM over 48 hours, demonstrates robust cytotoxic effects against various cancer cells, including lung, triple-negative breast, melanoma, and kidney cell lines, evidenced by apoptotic induction, cell cycle arrest in G2/M phase, and inhibition of microtubule protein polymerization. Notably, at concentrations of 0.1 µM, 0.25 µM, and 0.5 µM over 24 hours, it promotes dose-dependent apoptosis by enhancing cellular ROS production and significantly alters the cell cycle dynamics by increasing G2/M phase cell percentage (17.6% to 50.3%) while reducing G0/G1 phase cells (41.1% to 4.9%), corresponding to escalating concentrations. This compound’s pronounced cytotoxicity is quantified by varying IC50 values against the tested cell lines: 0.29 µM (A549), 1.48 µM (MDA-MB-231), 1.25 µM (B16-F10), 0.42 µM (BT-474), 0.49 µM (4 T1), and 1.58 µM (NRK-52E), showcasing its potential as an effective anti-cancer agent.