Type II topoisomerase inhibitor 1 is a potent and selective inhibitor of E. coli DNA gyrase with an IC50 value of 1.7 nM and forms hydrogen bonds with the Asp73 residue. It inhibits topoisomerase IV activity with an IC50 value of 0.98 μM and is applicable in the antibacterial area [1] [2].

Type II topoisomerase inhibitor 1 (13 e, 18 h) exhibits antibacterial efficacy against E. coli and S. aureus with minimum inhibitory concentrations (MIC) of 64 μg/mL and 16 μg/mL, respectively. At 50 μM over 48 hours, it inhibits HepG2 cell growth by 8.4%, showing inhibition rates of 1.5% and 8.4% in HepG2 cells at 10 μM and 50 μM, respectively.