2B4/CD244 Protein, Human, Recombinant (aa 22-221, His & Avi), Biotinylated

Catalog No. TMPK-00237

2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.

2B4/CD244 Protein, Human, Recombinant (aa 22-221, His & Avi), Biotinylated

Catalog No. TMPK-00237

Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Product Information

Biological Activity	Immobilized Biotinylated Human 2B4, His Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human CD48, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA.
Description	2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	Q9BZW8-2
Synonyms	SLAMF4,Nmrk,NKR2B4,NAIL,h2B4,CD244 antigen,CD244,2B4
Construction	Cys22-Arg221
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	24.9 kDa (predicted). Due to glycosylation, the protein migrates to 48-70 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	2B4 (CD244) is expressed by memory-phenotype CD8() T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.