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CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi)

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CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi)

Catalog No. TMPY-01973

CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.3 kDa and the accession number is A0A140HDP8.

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Pack Size	Price	Availability	Quantity
100 μg	$600	7-10 days
1 mg	$3,910	7-10 days

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Biological Description

Biological Information	1. Measured by its binding ability in a functional ELISA. Immobilized Rhesus CD16-AVI-His at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1The EC50 of biotinylated human IgG1 is 0.2-0.5 μg/ml. 2. Labeled biotin to CD16 / FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD16 / FCGR3 Protein, Rhesus, Recombinant (His & AVI Tag), Biotinylated bound to IgG1 Antibody was 0.1 μM.
Description	CD16/FCGR3 Protein, Rhesus, Recombinant (His & Avi) is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 25.3 kDa and the accession number is A0A140HDP8.
Species	Rhesus
Expression System	HEK293 Cells
Tag	His, Avi
Accession Number	A0A140HDP8
Synonyms	Fc receptor, IgG, low affinity III
Construction	The rhesus CD16 (NP_001258584.1) extracellular domain (Met 1- Gln 208) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE
Molecular Weight	25.3 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Fc receptors bind the most common class of antibody, IgG, are called Fc gamma receptors (FcγR). FcγR is divided into three classes, Fc γ RI (CD64), Fc γ RII (CD32), and Fc γ RIII (CD16). CD16 protein is a multifunctional, low/intermediate affinity receptor, which belongs to the immunoglobulin superfamily. It is found on the surface of natural killer cells, neutrophil polymorphonuclear leukocytes, monocytes and macrophages. Mouse CD16 is encoded by a single gene, while, human CD16 is expressed as two distinct forms (CD16a/FcγRIIIa and CD16b/FcγRIIIb) encoded by two different highly homologous genes in a cell type-specific manner. CD16 is involved in phagocytosis, secretion of enzymes, inflammatory mediators, antibody-dependent cellular cytotoxicity (ADCC), and clearance of immune complexes.