CD300A Protein, Human, Recombinant (Avi), Biotinylated

Catalog No. TMPK-00187

CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. CD300A Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 19.46 kDa and the accession number is Q9UGN4-1.

CD300A Protein, Human, Recombinant (Avi), Biotinylated

Catalog No. TMPK-00187

Pack Size	Price	Availability
100 μg	$932	7-10 days
500 μg	$3,720	7-10 days
1 mg	$6,210	7-10 days

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Product Information

Biological Activity	Immobilized Biotinylated Human CD300A, Avi Tag at 0.5μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Anti-CD300A Antibody, hFc Tag with the EC50 of 6.3ng/ml determined by ELISA.
Description	CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation. CD300A Protein, Human, Recombinant (Avi), Biotinylated is expressed in HEK293 mammalian cells with C-Avi tag. The predicted molecular weight is 19.46 kDa and the accession number is Q9UGN4-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-Avi
Accession Number	Q9UGN4-1
Synonyms	Pigr4,NKRL,Mcipr1,MAIR-I,LMIR1,IRp60,IRC1,IGSF12,CLM-8,CLM8,CD300a
Construction	Leu18-Pro180
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	19.46 kDa (predicted). Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.