CD31/PECAM-1 Protein, Human, Recombinant

Catalog No. TMPY-00707

CD31/PECAM-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 65.1 kDa and the accession number is EAW94208.1.

CD31/PECAM-1 Protein, Human, Recombinant

Catalog No. TMPY-00707

CD31/PECAM-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 65.1 kDa and the accession number is EAW94208.1.

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100 μg	$700	7-10 days

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Product Information

Biological Activity	Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8×104cells/well are added to CD31-coated plates (5μg/mL, 100 μL/well) in the presence of 10 μg/mL PHA,approximately >60% will adhere after 30 minutes at 37℃.
Description	CD31/PECAM-1 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 65.1 kDa and the accession number is EAW94208.1.
Species	Human
Expression System	HEK293 Cells
Tag	Tag Free
Accession Number	EAW94208.1
Synonyms	platelet/endothelial cell adhesion molecule 1,PECAM-1,PECA1,GPIIA',endoCAM,CD31/EndoCAM,CD31
Construction	The mature form of human CD31 (EAW94208.1) extracellular domain (Met1-Lys 601) with five additional amino acids (DDDDK) at the C-terminus was expressed and purified. Predicted N terminal: Gln 28
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	65.1 kDa (predicted); 115 kDa (reducing condition, due to glycosylation)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 100 mM Glycine, 10 mM NaCl, 50 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.