CD70 Protein, Human, Recombinant (mFc)

Pack Size	Price	Availability	Quantity
100 μg	$462	7-10 days
1 mg	$3,000	7-10 days

Pack Size

Price

Availability

Quantity

100 μg

$462

7-10 days

1 mg

$3,000

7-10 days

QWhether recombinant proteins be available?

AWhat species of protein do you need, and do you have any requirements for expression systems and tags? What experiments will the product be used for?

QHow to store the recombinant protein products?

A1. It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, they should be used promptly. If they cannot be used up within one week, the product can be stored at -20°C to -80°C for at least 3 months. 2. Avoid multiple freeze-thaw cycles and store products in aliquots. When aliquoting and freezing, it is advisable to use a solution or medium with a certain concentration of carrier protein for reconstitution. The product concentration should not be less than 100 μg/mL, and the volume per vial should be no less than 20 μL.

QWhy is the volume & weight of the product not as expected?

AThe lyophilized powder is obtained from the freeze-drying of protein solutions, and its weight is not equivalent to that of protein. The volume of powder is influenced by different factors, including the composition of the buffer prior to lyophilization, salt ion concentration, and the concentration of the product itself. Thus, it’s normal that the powder may appear plentiful or be skipped by the eye. Upon receiving the product, proceed with reconstitution, use, or aliquot storage as usual.

QHow are recombinant protein products shipped?

AMost recombinant proteins are delivered as lyophilized powder, which are insensitive to temperature change and are stable at room temperature. They are typically shipped at room temperature or with blue ice. For liquid recombinant proteins, dry ice is generally used for shipping. It is normal if the product is arrived with a melted ice pack. The protein can still be used without any negative impact on its quality for a short period. If a specific protein is unstable at room temperature, appropriate cold chain shipping will be arranged to ensure product quality remains intact.

QHow to reconstitute lyophilized recombinant proteins into a stock solution?

ACentrifuge the product at 10,000-12,000 rpm for 20-30 seconds before opening the vial to drive any protein that may be lodged in the cap or on the side of the tube to the bottom of the vial.In general, most proteins can be reconstituted by simply adding sterile distilled water. If other diluents are needed, they will be specified in the product's Certificate of Analysis (COA). It is recommended to prepare the stock solution with a concentration not lower than 100 μg/mL and aliquot for storage to avoid repeated freeze-thaw cycles.

Biological Activity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	CD70 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 43.8 kDa and the accession number is A0A0U5JA32.
Species	Human
Expression System	HEK293 Cells
Tag	N-mFc
Accession Number	P32970-1
Synonyms	TNFSF7,CD70 molecule,CD27LG,CD27L
Construction	A DNA sequence encoding the human CD70 (NP_001243.1) (Gln39-Pro193) was expressed with the Fc region of mouse IgG1 at the N-terminus. Predicted N terminal: Asp
Protein Purity	> 90 % as determined by SDS-PAGE.
Molecular Weight	43.8 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	CD70, a member of the tumor necrosis factor superfamily, is restricted to activated T-and B-lymphocytes and mature dendritic cells. Binding of CD70 to its receptor, CD27, is important in priming, effector functions, differentiation and memory formation of T-cells as well as plasma and memory B-cell generation. Tight control of CD70 expression is required to prevent lethal immunodeficiency. By selective transcription, CD70 is largely confined to activated lymphocytes and dendritic cells (DC). As a type II transmembrane receptor, CD70 is normally expressed on a subset of B, T and NK cells, where it plays a costimulatory role in immune cell activation. Immunohistochemical analysis of CD70 expression in multiple carcinoma types. The restricted expression pattern of CD70 in normal tissues and its widespread expression in various malignancies makes it an attractive target for antibody-based therapeutics. Investigations to exploit CD70 as a cancer target have lead to the identification of potential antibody-based clinical candidates.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint ProteinsImmune Checkpoint TargetsImmunotherapyTargeted Therapy

CD70 Protein, Human, Recombinant (mFc)

CD70 Protein, Human, Recombinant (mFc)

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