EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His)

Catalog No. TMPK-00207

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422.

EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His)

Catalog No. TMPK-00207

Pack Size	Price	Availability
100 μg	$302	7-10 days
500 μg	$1,210	7-10 days
1 mg	$2,010	7-10 days

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Product Information

Biological Activity	Immobilized Human EpCAM, His Tag at 0.2μg/ml (100μl/well) on the plate. Dose response curve for Anti-EpCAM Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.
Description	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 28.2 kDa and the accession number is P16422.
Species	Human
Expression System	HEK293 Cells
Tag	C-His
Accession Number	P16422
Synonyms	TROP1,TACSTD1,TACST-1,MOC31,MK1,MIC18,KSA,HNPCC8,gp40,ESA,EpCAM,EGP40,EGP314,EGP-2,EGP,DIAR5,CD326,ACSTD1
Construction	Gln24-Lys265
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	28.2 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.