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EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated

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EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated

Catalog No. TMPK-00205

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 30.1 kDa and the accession number is P16422.

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Pack Size	Price	Availability
100 μg	$814	7-10 days
500 μg	$3,250	7-10 days
1 mg	$5,440	7-10 days

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Biological Description

Biological Information	Immobilized Anti-EpCAM Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human EpCAM, His Tag with the EC50 of 14.4ng/ml determined by ELISA.
Description	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. EpCAM/TROP1 Protein, Human, Recombinant (aa 24-265, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 30.1 kDa and the accession number is P16422.
Species	Human
Expression System	HEK293 Cells
Tag	C-His-Avi
Accession Number	P16422
Synonyms	EGP40,TROP1,EGP314,MIC18,DIAR5,ESA,KSA,TACST-1,EpCAM,EGP,MOC31,MK1,EGP-2,ACSTD1,TACSTD1,CD326,HNPCC8,gp40
Construction	Gln24-Lys265
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	30.1 kDa (predicted). Due to glycosylation, the protein migrates to 30-45 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

Dose Conversion

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.

Keywords

MIC-18 MK 1 ACSTD-1 EGP-314 ACSTD 1 MOC 31 EGP 2 MK-1 HNPCC 8 CD 326 TROP-1 DIAR 5 TACSTD 1 MIC 18 TACST1 EGP 40 EGP-40 HNPCC-8 TROP 1 MOC-31 EGP2 CD-326 EGP 314 TACSTD-1 DIAR-5 TACST 1