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FCGR4 Protein, Mouse, Recombinant (His)

Catalog No. TMPY-01087
FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0.
Pack SizePriceAvailabilityQuantity
100 μg$386In Stock
200 μg$6827-10 days
500 μg$1,4407-10 days
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Product Information

Biological Activity
1.Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) . The EC50 of human IgG1 (Fc) is 0.11-0.25 μg/ml._x000D_ 2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag with an affinity constant of 3.43nM as determined in BLI assay(QC tested).
FCGR4 Protein, Mouse, Recombinant (His)FCGR4 Protein, Mouse, Recombinant (His)
Description
FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberA0A0B4J1G0
Synonyms
FcγRIV,Fcrl3,FcgRIV,Fcgr3a,FcgammaRIV,Fc receptor, IgG, low affinity IV,CD16-2,4833442P21Rik
Construction
The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 19
Protein Purity
> 95 % as determined by SDS-PAGE
FCGR4 Protein, Mouse, Recombinant (His)
Molecular Weight22.4 kDa (predicted); 25-35 kDa (reducing condition, due to glycosylation)
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution
A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.

Dose Conversion

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Tech Support

Please read the User Guide of Recombinant Proteins for more specific information.