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FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $386 | In Stock | |
200 μg | $682 | 7-10 days | |
500 μg | $1,440 | 7-10 days |
Biological Activity | 1.Measured by its binding ability in a functional ELISA. Immobilized mouse FCGR4 at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc) . The EC50 of human IgG1 (Fc) is 0.11-0.25 μg/ml._x000D_
2.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a on ProA Biosensor, can bind Recombinant Mouse FCGR4 Protein, His Tag with an affinity constant of 3.43nM as determined in BLI assay(QC tested). |
Description | FCGR4 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 22.4 kDa and the accession number is A0A0B4J1G0. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | A0A0B4J1G0 |
Synonyms | FcγRIV,Fcrl3,FcgRIV,Fcgr3a,FcgammaRIV,Fc receptor, IgG, low affinity IV,CD16-2,4833442P21Rik |
Construction | The amino acid sequence corronding to Met 1-Gln 203 of mouse FCGR4 (NP_653142.2) was fused with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 19 |
Protein Purity | > 95 % as determined by SDS-PAGE |
Molecular Weight | 22.4 kDa (predicted); 25-35 kDa (reducing condition, due to glycosylation) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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