FGF-1 Protein, Mouse, Rat, Recombinant

Catalog No. TMPY-01089

FGF-1 Protein, Mouse, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 kDa and the accession number is P61148.

FGF-1 Protein, Mouse, Rat, Recombinant

Catalog No. TMPY-01089

FGF-1 Protein, Mouse, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 kDa and the accession number is P61148.

Pack Size	Price	Availability
10 μg	$88	In Stock
100 μg	$306	7-10 days
1 mg	$2,000	7-10 days

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COA User Guide of Recombinant Proteins

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Product Information

Biological Activity	Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.04-0.2 ng/mL.
Description	FGF-1 Protein, Mouse, Rat, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 16 kDa and the accession number is P61148.
Species	Mouse,Rat
Expression System	E. coli
Tag	Tag Free
Accession Number	P61148
Synonyms	fibroblast growth factor 1 (acidic)
Construction	A DNA sequence encoding the mature form of mouse / rat FGF1 (NP_034327.1) (Phe16-Asp155) was expressed. Mouse and Rat FGF1 sequences are identical. Predicted N terminal: Met
Protein Purity	> 90 % as determined by SDS-PAGE
Molecular Weight	16 kDa (predicted); 16 kD (reducing conditions)
Endotoxin	Please contact us for more information.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 50 mM Na2SO4, 0.5 mM DTT, 1 mM EDTA, 20 mM Tris, pH 7.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	aFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.References