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Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. FLT3 Ligand Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.8 kDa and the accession number is P49771-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $511 | 7-10 days | |
500 μg | $2,040 | 7-10 days | |
1 mg | $3,410 | 7-10 days |
Biological Activity | Immobilized Human Human FLT3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. |
Description | Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. FLT3 Ligand Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 44.8 kDa and the accession number is P49771-1. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-hFc |
Accession Number | P49771-1 |
Synonyms | SL cytokine,FLT3LG,Flt3L,Flt3 ligand,FL |
Construction | Thr27-Pro185 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | 44.8 kDa (predicted). Due to glycosylation, the protein migrates to 90-118 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, a mixture containing 8% trehalose and 0.01% Tween is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. |
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