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Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. FLT3 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 84.78 kDa and the accession number is Q00342.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $487 | 7-10 days | |
500 μg | $1,950 | 7-10 days | |
1 mg | $3,250 | 7-10 days |
Biological Activity | Human FLT3 Ligand, hFc Tag immobilized on CM5 Chip can bind Mouse FLT3, hFc Tag with an affinity constant of 35.07 nM as determined in SPR assay. |
Description | Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. FLT3 Protein, Mouse, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 84.78 kDa and the accession number is Q00342. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-hFc |
Accession Number | Q00342 |
Synonyms | SL cytokine,FLT3LG,Flt3L,Flt3 ligand,FL |
Construction | Asn28-Ser544 |
Protein Purity | > 95% as determined by Tris-Bis PAGE |
Molecular Weight | 84.78 kDa (predicted). Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Flt‑3 Ligand, also known as FL, is an alpha -helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages.Stimulates the proliferation of early hematopoietic cells by activating FLT3. Synergizes well with a number of other colony stimulating factors and interleukins. |
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