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IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $106 | In Stock | |
20 μg | $165 | 7-10 days | |
50 μg | $290 | 7-10 days | |
100 μg | $447 | 7-10 days | |
200 μg | $791 | 7-10 days | |
500 μg | $1,670 | 7-10 days |
Biological Activity | Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 0.2-2 ng/mL. |
Description | IL-12 Protein, Human, Recombinant is expressed in HEK293 mammalian cells. The predicted molecular weight is 57.2 kDa and the accession number is P29459-1&NP_002178.2. |
Species | Human |
Expression System | HEK293 Cells |
Tag | Tag Free |
Accession Number | P29459-1&NP_002178.2 |
Synonyms | P35,NKSF2,NKSF1,NKSF,NFSK,interleukin 12A,IMD29,IMD28,IL-12B,IL-12A,IL12 p70,IL12,CLMF2,CLMF |
Construction | A DNA sequence encoding the human IL12A (P29459-1)(Met1-Ser219) was expressed, constructed the plasmid 1; A DNA sequence encoding the human IL12B (NP_002178.2)(Met1-Ser328) was expressed, constructed the plasmid 2. The two plasmids were co-expressed and the human IL12A&IL12B heterodimer was purified. Predicted N terminal: Arg23&Ile23 |
Protein Purity | ≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC. |
Molecular Weight | 57.2 kDa (predicted); 34 & 41 kDa (reducing conditions) |
Endotoxin | < 5 EU/mg of the protein. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
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