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Pack Size | Price | Availability | Quantity |
---|---|---|---|
20 μg | $244 | 7-10 days | |
100 μg | $695 | 7-10 days | |
1 mg | $4,020 | 7-10 days |
Biological Information | 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL4-His at 10 μg/mL (100 μl/well) can bind rat IL4R-Fc , The EC50 of rat IL4R-Fc is 0.12-0.29 μg/mL.
2. Measured by its ability to inhibit mIL4-dependent proliferation of HT-2 cells. The ED50 for this effect is typically 2-10 ng/ml in the presence of 2 ng/ml Recombinant Mouse IL4. |
Description | IL-4 Protein, Mouse, Recombinant (Q136D, Y139D, His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 14.9 kDa and the accession number is P07750. |
Species | Mouse |
Expression System | Baculovirus Insect Cells |
Tag | C-His |
Accession Number | P07750 |
Synonyms | interleukin 4,BSF-1,Il-4 |
Construction | The Mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus. |
Protein Purity | > 95 % as determined by SDS-PAGE |
Molecular Weight | 14.9 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.5. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Interleukin-4, also known as IL4, is a secreted protein that belongs to the IL-4 / IL-13 family. Interleukin-4 / IL4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation. It enhances both secretion and cell surface expression of IgE and IgG1. Interleukin-4 / IL4 also regulates the expression of the low-affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Interleukin-4 is essential for the switching of B cells to IgE antibody production and the maturation of T helper (Th) cells toward the Th2 phenotype. It participates in at least several B-cell activation processes as well as other cell types. However, studies show that double mutant (Q116D, Y119D) of the murine IL4 protein (QY), both glutamine 116 and tyrosine 119, which binds to the IL4 receptor alpha, completely inhibits in a dose-dependent manner the IL4-induced proliferation of lipopolysaccharide-stimulated murine splenic B-cells, of the murine T cell line CTLL-2, and the murine pre-B-cell line BA/F3. QY also inhibited the IL4-stimulated up-regulation of CD23 expression by lipopolysaccharide-stimulated murine splenic B-cells and abolished tyrosine phosphorylation of the transcription factor Stat6 and the tyrosine kinase Jak3 in IL4-stimulated BA/F3 cells.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy |
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