1. Loaded Cynomolgus IL-4RA-His on HIS1K Biosensor, can bind Human IL-4-Fc with an affinity constant of <10^-3 nM as determined in BLI assay. (Regularly tested). 2. Immobilized Human IL-4-Fc at 5μg/ml (100 μl/well) can bind Cynomolgus IL-4Ra-His . The ED50 of Recombinant Human IL-4Ra-His is 79.49 ng/ml. (Regularly tested);

IL-4 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-Fc tag. The predicted molecular weight is 40-55 KDa and the accession number is P05112.

Human

HEK293 Cells

Lymphocyte Stimulatory Factor 1,BSF-1,Pitrakinra,IL-4,IL4,Interleukin-4,Binetrakin,B-Cell Stimulatory Factor 1

His25-Ser153

His25-Ser153

Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.

Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.