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Pack Size | Price | Availability | Quantity |
---|---|---|---|
10 μg | $83 | In Stock | |
50 μg | $250 | 7-10 days | |
500 μg | $1,220 | 7-10 days | |
1 mg | $2,730 | 7-10 days |
Biological Information | 1. Loaded Cynomolgus IL-4RA-Fc on Protein A Biosensor, can bind Human IL-4-His with an affinity constant of 0.33 nM as determined in BLI assay. (Regularly tested).
2. Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. The ED50 for this effect is 0.05-0.5 ng/ml. (QC verified); ![]() ![]() |
Description | IL-4 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with C-6xHis tag. The predicted molecular weight is 18 KDa and the accession number is P05112. |
Species | Human |
Expression System | HEK293 Cells |
Tag | C-6xHis |
Accession Number | P05112 |
Synonyms | Pitrakinra,Binetrakin,B-Cell Stimulatory Factor 1,IL4,Interleukin-4,IL-4,BSF-1,Lymphocyte Stimulatory Factor 1 |
Amino Acid | His25-Ser153 |
Construction | His25-Ser153 |
Protein Purity | Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC. ![]() ![]() |
Molecular Weight | 18 KDa (reducing condition) |
Endotoxin | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
Research Background | Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response. |
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