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MSLN/Mesothelin Protein (Primary Amine Labeling), Mouse, Recombinant (His), Biotinylated

Catalog No. TMPK-01026
MSLN/Mesothelin Protein (Primary Amine Labeling), Mouse, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 35.2 kDa and the accession number is Q61468-1.
Pack SizePriceAvailabilityQuantity
100 μg$8147-10 days
500 μg$3,2507-10 days
1 mg$5,4407-10 days
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Product Information

Biological Activity
Immobilized Human CA125, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse MSLN, His Tag with the EC50 of 0.58μg/ml determined by ELISA.
Description
MSLN/Mesothelin Protein (Primary Amine Labeling), Mouse, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 35.2 kDa and the accession number is Q61468-1.
Species
Mouse
Expression System
HEK293 Cells
TagC-His
Accession NumberQ61468-1
Synonyms
SMR,MSLN,MPFSMRP,MPF,Mesothelin,CAK1
Construction
Asp298-Ser600
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 90% as determined by HPLC
Molecular Weight35.2 kDa (predicted). Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result.
Endotoxin< 1 EU/μg by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice.
Research Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

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