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MSLN/Mesothelin Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 58.6 kDa and the accession number is A0A2K5TW94-1.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $487 | 7-10 days | |
500 μg | $1,950 | 7-10 days | |
1 mg | $3,250 | 7-10 days |
Biological Activity | Immobilized Cynomolgus MSLN, hFc Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-MSLN Antibody, hFc Tag with the EC50 of 35.6ng/ml determined by ELISA (QC Test). |
Description | MSLN/Mesothelin Protein, Cynomolgus, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 58.6 kDa and the accession number is A0A2K5TW94-1. |
Species | Cynomolgus |
Expression System | HEK293 Cells |
Tag | C-hFc |
Accession Number | A0A2K5TW94-1 |
Synonyms | SMR,MSLN,MPFSMRP,MPF,Mesothelin,CAK1 antigen,CAK1 |
Construction | Asp296-Gly580 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 58.6 kDa (predicted). Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
Reconstitution | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally. |
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