P3H 1 Protein, Streptomyces sp., Recombinant (His & Myc)

Catalog No. TMPH-03606

Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp). D-proline, trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, cis-4-hydroxy-D-proline, and 3,4-dehydro-DL-proline are not substrates. P3H 1 Protein, Streptomyces sp., Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 44.0 kDa and the accession number is P96010.

P3H 1 Protein, Streptomyces sp., Recombinant (His & Myc)

Catalog No. TMPH-03606

Pack Size	Price	Availability
20 μg	$360	20 days
100 μg	$678	20 days
1 mg	$2,300	20 days

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User Guide of Recombinant Proteins

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Product Information

Biological Activity	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp). D-proline, trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, cis-4-hydroxy-D-proline, and 3,4-dehydro-DL-proline are not substrates. P3H 1 Protein, Streptomyces sp., Recombinant (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 44.0 kDa and the accession number is P96010.
Species	Streptomyces
Expression System	E. coli
Tag	N-10xHis, C-Myc
Accession Number	P96010
Synonyms	Proline 3-hydroxylase type I,Proline 3-hydroxylase 1,L-proline cis-3-hydroxylase 1
Amino Acid	MRSHILGRIELDQERLGRDLEYLATVPTVEEEYDEFSNGFWKNIPLYNASGGSEDRLYRDLEGSPAQPTKHAEQVPYLNEIITTVYNGERLQMARTRNLKNAVVIPHRDFVELDRELDQYFRTHLMLEDSPLAFHSDDDTVIHMRAGEIWFLDAAAVHSAVNFAEFSRQSLCVDLAFDGAFDEKEAFADATVYAPNLSPDVRERKPFTKEREAGILALSGVIGRENFRDILFLLSKVHYTYDVHPGETFEWLVSVSKGAGDDKMVEKAERIRDFAIGARALGERFSLTTW
Construction	1-290 aa
Protein Purity	> 90% as determined by SDS-PAGE.
Molecular Weight	44.0 kDa (predicted)
Formulation	If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution	Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background	Dioxygenase that catalyzes the 2-oxoglutarate-dependent selective hydroxylation of free L-proline to cis-3-hydroxy-L-proline (cis-3-Hyp). D-proline, trans-4-hydroxy-L-proline, cis-4-hydroxy-L-proline, cis-4-hydroxy-D-proline, and 3,4-dehydro-DL-proline are not substrates.

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