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B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 Protein (Primary Amine Labeling), Cynomolgus/Rhesus macaque, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 26 kDa and the accession number is G7PSE7.
Pack Size | Price | Availability | Quantity |
---|---|---|---|
100 μg | $814 | 7-10 days | |
500 μg | $3,250 | 7-10 days | |
1 mg | $5,440 | 7-10 days |
Biological Activity | Immobilized Biotinylated Cynomolgus/Rhesus macaque PD-L1, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-PD-L1 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA. |
Description | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. PD-L1 Protein (Primary Amine Labeling), Cynomolgus/Rhesus macaque, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 26 kDa and the accession number is G7PSE7. |
Species | Cynomolgus,Rhesus |
Expression System | HEK293 Cells |
Tag | C-His |
Accession Number | G7PSE7 |
Synonyms | PD-L1B7 homolog 1,PD-L1,PDL1,PDCD1LG1,PDCD1L1,CD274,B7-H1,B7H1,B7-H |
Construction | Phe19-Arg238 |
Protein Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Molecular Weight | 26 kDa (predicted). Due to glycosylation, the protein migrates to 35-40 kDa based on Tris-Bis PAGE result. |
Endotoxin | < 1 EU/μg by the LAL method. |
Formulation | Supplied as 0.22 μm filtered solution in PBS (pH 7.4). |
Stability & Storage | It is recommended to store the product under sterile conditions at -70°C or lower. Samples are stable for up to 12 months at -80°C. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | Shipping with blue ice. |
Research Background | B7-H1, also known as PD-L1 and CD274, is an approximately 65 kDa transmembrane glycoprotein in the B7 family of immune regulatory molecules. PD-L1 has been identified as the ligand for the immunoinhibitory receptor programmed death-1(PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. |
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