1.Measured by its binding ability in a functional ELISA. 2. Immobilized biotinylated human PDGFRb-His at 10μg/mL (100μL/well) can bind Cynomolgus PDGFB-Fc, The EC50 of Cynomolgus PDGFB-Fc is 30-80ng/mL.

PDGFRB Protein, Human, Recombinant (His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.6 kDa and the accession number is P09619-1.

Human

HEK293 Cells

platelet-derived growth factor receptor, β polypeptide,platelet-derived growth factor receptor, beta polypeptide,PENTT,PDGFR-1,PDGFR1,PDGFR,KOGS,JTK12,IMF1,IBGC4,CD140B

A DNA sequence encoding the human PDGFRβ (NP_002600.1) extracellular domain (Met1-Lys531) was expressed, with a C-terminal polyhistidine tag. The expressed protein was biotinylated in vitro. Predicted N terminal: Leu 33

> 90 % as determined by SDS-PAGE

A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

The cluster of differentiation (CD) system is commonly used as cell markers in Immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF)-B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.Cancer ImmunotherapyImmune CheckpointImmunotherapyTargeted Therapy