SEMA4D Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-01379

Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 80.1 kDa and the accession number is A0A2K5TZC9.

SEMA4D Protein, Cynomolgus, Recombinant (His)

Catalog No. TMPK-01379

Pack Size	Price	Availability
100 μg	$324	7-10 days
500 μg	$1,300	7-10 days
1 mg	$2,170	7-10 days

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Product Information

Biological Activity	Immobilized Cynomolgus Semaphorin 4D, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-Semaphorin 4D Antibody , hFc Tag with the EC50 of 11.8ng/ml determined by ELISA.
Description	Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression. SEMA4D Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with C-His tag. The predicted molecular weight is 80.1 kDa and the accession number is A0A2K5TZC9.
Species	Cynomolgus
Expression System	HEK293 Cells
Tag	C-His
Accession Number	A0A2K5TZC9
Synonyms	Semaphorin-4D,SEMAJ,SEMA4D,M-sema-G,MGC169141,MGC169138,FLJ46484,FLJ39737,FLJ34282,FLJ33485,coll-4,CD100,C9orf164
Construction	Phe24-Arg734
Protein Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Molecular Weight	80.1 kDa (predicted). Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Endotoxin	< 1 EU/μg by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
Reconstitution	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	Semaphorin 4D (Sema4D) is a multifunctional protein widely expressed in an organism that plays an important role in the control of many physiological and pathological processes, including immunoregulation, neurogenesis, angiogenesis, and tumor progression.