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TIGIT Protein, Human, Recombinant (mFc)

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TIGIT Protein, Human, Recombinant (mFc)

Catalog No. TMPY-06134

TIGIT Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 39.4 kDa and the accession number is Q495A1-1.

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Pack Size	Price	Availability	Quantity
50 μg	$357	7-10 days
500 μg	$2,330	7-10 days

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Biological Description

Biological Information	Immobilized Human TIGIT(mFc Tag) at 2 μg/mL (100 μL/well) can bind Human CD155/PVR (hFc & AVI Tag), Biotinylated, the EC50 of Human CD155/PVR (hFc & AVI Tag), Biotinylated is 15-120 ng/mL.
Description	TIGIT Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 39.4 kDa and the accession number is Q495A1-1.
Species	Human
Expression System	HEK293 Cells
Tag	C-mFc
Accession Number	Q495A1-1
Synonyms	VSTM3,VSIG9,WUCAM,T cell immunoreceptor with Ig and ITIM domains
Construction	The Human TIGIT (NP_776160.2) (Met1-Pro141) was expressed with the Fc region of Mouse IgG2a at the C-terminus.
Protein Purity	> 95 % as determined by SDS-PAGE.
Molecular Weight	39.4 kDa (predicted)
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Stability & Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
Shipping	In general, Lyophilized powders are shipping with blue ice.
Research Background	TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy