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Pack Size | Price | Availability | Quantity |
---|---|---|---|
50 μg | $357 | 7-10 days | |
1 mg | $3,910 | 7-10 days |
Biological Information | Immobilized Mouse CD155/PVR Protein, Recombinant (His Tag) at 2μg/mL (100μL/well) can bind TIGIT Protein, Mouse, Recombinant (mFc Tag), the EC50 of TIGIT Protein, Mouse, Recombinant (mFc Tag) is 10-50 ng/mL. |
Description | TIGIT Protein, Mouse, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 39.93 kDa and the accession number is NP_001139797.1. |
Species | Mouse |
Expression System | HEK293 Cells |
Tag | C-mFc |
Accession Number | NP_001139797.1 |
Synonyms | ENSMUSG00000071552,T cell immunoreceptor with Ig and ITIM domains,Vstm3 |
Construction | The Mouse TIGIT (NP_001139797.1) (Met1-Gly141) was expressed with the Fc region of Mouse IgG2a at the C-terminus. |
Protein Purity | > 95 % as determined by SDS-PAGE. |
Molecular Weight | 39.93 kDa (predicted) |
Endotoxin | < 1.0 EU/μg of the protein as determined by the LAL method. |
Formulation | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
Reconstitution | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
Stability & Storage | It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
Shipping | In general, Lyophilized powders are shipping with blue ice. |
Research Background | TIGIT, also known as V-set and transmembrane domain-containing protein 3 (VSTM3) or V-set and immunoglobulin domain-containing protein 9 (VSIG9) is a new surface protein containing an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on regulatory, memory, activated T cells and NK cells. It binds PVR with high affinity, and PVRL2 with lower affinity, but not PVRL3. Knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses, however, TIGIT inhibits NK cytotoxicity directly through its ITIM. TIGIT suppresses T cell activation by promoting the generation of mature immunoregulatory dendritic cells. The binding of PVR to TIGIT on human dendritic cells enhanced the production of IL-1 and diminished the production of IL-12p4. Also, TIGIT counter inhibits the NK-mediated killing of tumor cells and protects normal cells from NK-mediated cytotoxicity thus providing an "alternative self" mechanism for MHC class I inhibition.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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