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Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc)

Catalog No. TMPH-03658

Envelope protein that binds to heparan sulfate on the cell surface and might provide virion attachment to target cell. Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 40.3 kDa and the accession number is P20497.

Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc)

Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc)

Catalog No. TMPH-03658
Envelope protein that binds to heparan sulfate on the cell surface and might provide virion attachment to target cell. Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 40.3 kDa and the accession number is P20497.
Pack SizePriceAvailabilityQuantity
20 μg$36020 days
100 μg$67820 days
1 mg$2,30020 days
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Product Information

Biological Activity
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
Envelope protein that binds to heparan sulfate on the cell surface and might provide virion attachment to target cell. Vaccinia virus (strain Copenhagen) Ag35 Protein (His & Myc) is expressed in E. coli expression system with N-10xHis and C-Myc tag. The predicted molecular weight is 40.3 kDa and the accession number is P20497.
Species
VACV
Expression System
E. coli
TagN-10xHis, C-Myc
Accession NumberP20497
Synonyms
Virion envelope protein p35,OPG108,Envelope protein H3,Ag35
Amino Acid
MAAVKTPVIVVPVIDRPPSETFPNVHEHINDQKFDDVKDNEVMPEKRNVVVVKDDPDHYKDYAFIQWTGGNIRNDDKYTHFFSGFCNTMCTEETKRNIARHLALWDSNFFTELENKKVEYVVIVENDNVIEDITFLRPVLKAMHDKKIDILQMREIITGNKVKTELVMDKNHTIFTYTGGYDVSLSAYIIRVTTALNIVDEIIKSGGLSSGFYFEIARIENEMKINRQILDNAAKYVEHDPRLVAEHRFENMKPNFWSRIGTAAAKRYPGVMYAFTTPLISFFG
Construction
1-284 aa
Protein Purity
> 85% as determined by SDS-PAGE.
Molecular Weight40.3 kDa (predicted)
FormulationIf the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
Stability & Storage
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
ShippingIn general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
Research Background
Envelope protein that binds to heparan sulfate on the cell surface and might provide virion attachment to target cell.

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Please read the User Guide of Recombinant Proteins for more specific information.