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(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane

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Catalog No. T35456Cas No. 257641-01-3

(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane (BMC) is a cyclohexane with two mercaptoacetamido groups. It is used, both in vitro and in vivo, to promote the correct folding of proteins that require disulfide bonds for functionality. For in vitro applications, BMC may be used to facilitate protein refolding of solubilized proteins from inclusion bodies. Reducing agents, like dithiothreitol, may interfere with refolding. BMC may also be added to the growth medium of cells, including yeast cells, to enhance the folding of proteins with disulfide bonds in vivo.

(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane

(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane

😃Good
Catalog No. T35456Cas No. 257641-01-3
(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane (BMC) is a cyclohexane with two mercaptoacetamido groups. It is used, both in vitro and in vivo, to promote the correct folding of proteins that require disulfide bonds for functionality. For in vitro applications, BMC may be used to facilitate protein refolding of solubilized proteins from inclusion bodies. Reducing agents, like dithiothreitol, may interfere with refolding. BMC may also be added to the growth medium of cells, including yeast cells, to enhance the folding of proteins with disulfide bonds in vivo.
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10 mg$110 35 days
50 mg$483 35 days
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Product Introduction

Bioactivity
Description
(±)-trans-1,2-Bis(2-mercaptoacetamido)cyclohexane (BMC) is a cyclohexane with two mercaptoacetamido groups. It is used, both in vitro and in vivo, to promote the correct folding of proteins that require disulfide bonds for functionality. For in vitro applications, BMC may be used to facilitate protein refolding of solubilized proteins from inclusion bodies. Reducing agents, like dithiothreitol, may interfere with refolding. BMC may also be added to the growth medium of cells, including yeast cells, to enhance the folding of proteins with disulfide bonds in vivo.
Chemical Properties
Molecular Weight262.39
FormulaC10H18N2O2S2
Cas No.257641-01-3
Storage & Solubility Information
StoragePowder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
DMSO:PBS (pH 7.2) (1:1): 0.5 mg/mL (1.91 mM), Sonication is recommended.
DMF: 20 mg/mL (76.22 mM), Sonication is recommended.
DMSO: 20 mg/mL (76.22 mM), Sonication is recommended.
Ethanol: 2 mg/mL (7.62 mM), Sonication is recommended.
Solution Preparation Table
Ethanol/DMF/DMSO
1mg5mg10mg50mg
5 mM0.7622 mL3.8111 mL7.6222 mL38.1112 mL
DMF/DMSO
1mg5mg10mg50mg
10 mM0.3811 mL1.9056 mL3.8111 mL19.0556 mL
20 mM0.1906 mL0.9528 mL1.9056 mL9.5278 mL
50 mM0.0762 mL0.3811 mL0.7622 mL3.8111 mL

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Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:
TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.
1 Enter information below:
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2 Enter the in vivo formulation:
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Keywords

(±) trans 1,2 Bis(2 mercaptoacetamido)cyclohexanetrans-1,2-Bis(2-mercaptoacetamido)cyclohexane(±)trans1,2Bis(2mercaptoacetamido)cyclohexane
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