Shopping Cart
- Remove All
![TargetMol](https://www.targetmol.com/storage/error/oops.webp)
Your shopping cart is currently empty
Pack Size | Price | Availability | Quantity |
---|---|---|---|
1 mg | $37 | In Stock | |
5 mg | $97 | In Stock | |
10 mg | $155 | In Stock | |
25 mg | $313 | In Stock | |
50 mg | $471 | In Stock | |
100 mg | $710 | In Stock | |
500 mg | $1,490 | In Stock | |
1 mL x 10 mM (in DMSO) | $98 | In Stock |
Description | 2-NBDG is a fluorescent indicator for direct glucose uptake measurement. It is an indicator of cell viability. |
In vitro | METHODS: Flow cytometry was used to detect glucose uptake: 1, Cells were seeded at 1*104/well in 96-well plates, and the experiment was performed within 24-48 h. The cells were incubated at 37 ℃ for 10-180 min. 2, Remove the cell culture medium, add fresh medium containing 2-NBDG (5-40 µM), and incubate for 10-180 min at 37 ℃. 3. Remove the medium and wash twice with pre-cooled PBS to stop the 2-NBDG uptake reaction. Resuspend in fresh medium and perform flow cytometry within 30 min. [1] |
In vivo | METHODS: Glucose uptake by circulating breast cancer cells was detected by fluorescent microscopy: 1. A mouse blood sample (100 µL/mouse) was collected by puncturing the mouse saphenous vein. 2. Incubate the blood sample containing circulating breast cancer cells with 2-NBDG (5 µg/100 µL blood) for 30 min at 37℃ in a dark incubator. 3. Add the magnetic bead suspension (1µL 1%) to 100 µL of blood sample and incubate for 30 min at 4°C with gentle shaking to promote binding of the magnetic beads to the circulating breast cancer cells. 4. Separate the circulating breast cancer cells from the blood using a magnetic separation rack, wash with PBS 3 times, resuspend in 100 µL PBS and transfer to a 96-well cell plate. 5. 2-NBDG uptake by circulating breast cancer cells was examined under a fluorescence microscope equipped with a 488 nm filter. Large cells with a fluorescent signal derived from fluorescent 2-NBDG uptake by cells were counted as hypermetabolic circulating breast cancer cells, and small-sized normal mouse blood cells (lymphocytes and RBCs) showed no or little 2-NBDG fluorescent signal. [2] |
Molecular Weight | 342.26 |
Formula | C12H14N4O8 |
Cas No. | 186689-07-6 |
Storage | store at low temperature,keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice. | ||||||||||||||||||||
Solubility Information | H2O: 5 mg/mL (14.61 mM), Sonication and heating to 60℃ are recommended. | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
H2O
|
Copyright © 2015-2024 TargetMol Chemicals Inc. All Rights Reserved.