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3X (DYKDDDDK) Peptide

Catalog No. TP2174

The DYKDDDDK-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. The DYKDDDDK peptide binds to the antibody M1. To improve the detection of the DYKDDDDK tag the 3x (DYKDDDDK) system has been developed. This three tandem DYKDDDDK epitope is hydrophilic, 22-amino-acids long, and can detect up to 10 fmol of the expressed fusion protein. The DYKDDDDK-tagged maltodextrin-binding protein of Pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.

3X (DYKDDDDK) Peptide

3X (DYKDDDDK) Peptide

Catalog No. TP2174
The DYKDDDDK-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. The DYKDDDDK peptide binds to the antibody M1. To improve the detection of the DYKDDDDK tag the 3x (DYKDDDDK) system has been developed. This three tandem DYKDDDDK epitope is hydrophilic, 22-amino-acids long, and can detect up to 10 fmol of the expressed fusion protein. The DYKDDDDK-tagged maltodextrin-binding protein of Pyrococcus furiosus has been crystallized4 and the quality of the crystals was very similar to that of crystals of untagged protein.
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Product Introduction

Bioactivity
Description
The DYKDDDDK-tag system utilizes a short, hydrophilic 8- amino-acid peptide that is fused to the protein of interest1. The DYKDDDDK peptide binds to the antibody M1. To improve the detection of the DYKDDDDK tag the 3x (DYKDDDDK) system has been developed.
Chemical Properties
Molecular Weight2861.87
FormulaC120H169N31O49S
Storage & Solubility Information
Storagekeep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
Solubility Information
TBS (0.5M Tris-HCl, pH 7.4, with 1M NaCl): ≥25 mg/mL

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TargetMol | Animal experimentsFor example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。
Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSOTargetMol | reagent (mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.
Preparation method for in vivo formula: Take 50 μL DMSOTargetMol | reagent main solution, add 300 μLPEG300TargetMol | reagent mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2OTargetMol | reagent mix well and clarify
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3X (DYKDDDDK) Peptide
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