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4-Nitrophenyl α-D-galactopyranoside

Name: 4-Nitrophenyl α-D-galactopyranoside
Brand: TargetMol
SKU: T19139
Price: 30 USD
Availability: InStock
Rating: 5 (10 reviews)

4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside used for detecting α-galactosidase activity. The amount of released pNP increases significantly when PNP-alpha-D-Gal is used as a substrate.

Catalog No. T19139Cas No. 7493-95-0

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Purity:99.44%

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COA HNMR HPLC

4-Nitrophenyl α-D-galactopyranoside

Catalog No. T19139Alias PNP-α-D-Gal, PNP-alpha-D-GalCas No. 7493-95-0

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Pack Size	Price	Availability	Quantity
100 mg	$30	In Stock
1 mL x 10 mM (in DMSO)	$39	In Stock

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Product Introduction

Bioactivity

Description	4-Nitrophenyl α-D-galactopyranoside (PNP-alpha-D-Gal) is an artificial substrate of 4-nitrophenyl (pNP) glycopyranoside used for detecting α-galactosidase activity. The amount of released pNP increases significantly when PNP-alpha-D-Gal is used as a substrate.
In vitro	Using 4-nitrophenol (pNP)-glycopyranosides as pseudosubstrates, the amount of liberated pNP from the 4-Nitrophenyl α-D-galactopyranoside is significantly higher than that from the other pNP-glycopyranosides at pH 4.0 while no activity was detected at pH 7.0. The digestion assays using pNP glycopyranosides as artificial substrates exhibit that yielding liberated galactose residues from 4-Nitrophenyl α-D-galactopyranoside mainly at pH 4.0, i.e. the pH level where yield threshold is decreased at most.
Alias	PNP-α-D-Gal, PNP-alpha-D-Gal

Chemical Properties

Molecular Weight	301.25
Formula	C₁₂H₁₅NO₈
Cas No.	7493-95-0

Storage & Solubility Information

Storage

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

Solubility Information

DMSO: 50 mg/mL (165.98 mM)

Solution Preparation Table

DMSO

	1mg	5mg	10mg	50mg
1 mM	3.3195 mL	16.5975 mL	33.1950 mL	165.9751 mL
5 mM	0.6639 mL	3.3195 mL	6.6390 mL	33.1950 mL
10 mM	0.3320 mL	1.6598 mL	3.3195 mL	16.5975 mL
20 mM	0.1660 mL	0.8299 mL	1.6598 mL	8.2988 mL
50 mM	0.0664 mL	0.3320 mL	0.6639 mL	3.3195 mL
100 mM	0.0332 mL	0.1660 mL	0.3320 mL	1.6598 mL

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In Vivo Formulation Calculator (Clear solution)

Please enter your animal experiment information in the following box and click Calculate to obtain the mother liquor preparation method and in vivo formula preparation method:

For example, your dosage is 10 mg/kg Each animal weighs 20 g, and the dosage volume is 100 μL . TargetMol | Animal experiments

A total of 10 animals were administered, and the formula you used is 5% TargetMol | reagent

DMSO+30% PEG300+5% Tween 80+60% ddH2O. So your working solution concentration is 2 mg/mL。

Mother liquor preparation method: 2 mg of drug dissolved in 50 μL DMSO TargetMol | reagent

(mother liquor concentration of 40 mg/mL), if you need to configure a concentration that exceeds the solubility of the product, please contact us first.

Preparation method for in vivo formula: Take 50 μL DMSO TargetMol | reagent

main solution, add 300 μLPEG300 TargetMol | reagent

mix well and clarify, then add 50 more μL Tween 80, mix well and clarify, then add 600 more μLddH2O TargetMol | reagent

mix well and clarify

For Reference Only. Please develop an appropriate dissolution method based on your laboratory animals and route of administration.

Dose Conversion

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Please see Inhibitor Handling Instructions for more frequently ask questions. Topics include: how to prepare stock solutions, how to store products, and cautions on cell-based assays & animal experiments, etc

Keywords

4-nitrophenylPNP-a-D-GalInhibitoryieldin4 Nitrophenyl α D galactopyranoside4-Nitrophenyl a-D-galactopyranosideacidic-pHglycopyranoside4-Nitrophenyl alpha-D-galactopyranosideα-galactosidaseartificial4Nitrophenyl αDgalactopyranosidesubstrateinhibit4-Nitrophenyl α-D-galactopyranoside

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